Document Type : Review articles
Authors
1
Department of Microbiology Research Group, Faculty of Food Industry and Agriculture, Standard Research Institute, Institute of Standard and Industrial Research of Iran, Karaj, Iran
2
Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3
Department of Food Technology, Faculty of Nutrition Sciences and Food Technology/National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background and objective: Gelatin is used as an important component in the structure of food and medicine due to its special properties. The gelatin used in industry is mainly produced from animal species of bovine and porcine. In some cases, to reduce the cost of the product, a substandard raw material or other than what is stated in the product labeling is used. This is especially important in cases where the health of the consumer is endangered or when the use of certain substances is contrary to the religious precepts and beliefs of the community. Therefore, the ability to identify, detect and, in a more comprehensive way, authenticate the components of food with the origin of living organisms is essential for the health control system of the country. Identification of the species used in the gelatin structure is necessary from the perspective of consumer confidence in food health safety as well as religious beliefs.
Results: Several methods have been introduced to determine the species in gelatin and its products from around the world, each has its advantages and disadvantages. In this research, the physical, chemical, and biological analytical methods used to identify the origin of gelatin and the authentication of the Halal product have been investigated. Methods such as FTIR spectroscopy, chromatography, ELISA, and polymerase chain reaction have been used for this purpose. One of the most successful methods is the PCR and its developed methods that can detect, identify and differentiate close species with sensitivity, specificity, high speed, and low-cost process.
Conclusion: The validated DNA based methods such as PCR are more efficient than other methods and they can be used as reliable methods with 99% confidence, with a low detection limit (about nanograms of DNA per gram of sample). of course, specificity, sensitivity, robustness, reproducibility and repeatability of test method must be tested.
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